ABSTRACT
Ferroptosis is an iron-dependent programmed cell death characterized by reactive oxygen species-induced lipid peroxide accumulation, which is different from cell apoptosis, pyroptosis, necrosis or autophagy. Ferroptosis plays an important role in the regulation of tumorigenesis and tumor development. Recent studies have shown that natural medicinal ingredients can induce ferroptosis in tumor cells through glutathione (GSH)/glutathione peroxidase 4 (GPx4) pathway, iron metabolism, lipid metabolism or other mechanisms. It has been reported that more than 30 natural medicinal ingredients can induce ferroptosis in tumor cells with multiple pathways and multiple targets. This article reviews the current research progress on the antitumor effects of natural medicinal ingredients through inducing cell ferroptosis.
Subject(s)
Humans , Apoptosis , Autophagy , Ferroptosis , Neoplasms/drug therapy , Reactive Oxygen SpeciesABSTRACT
Pace of life and work of people is accelerating nowadays, and hospitals keep improving their services, which gives rise to the decoction and delivery service from the third party vendors for traditional Chinese medicine(TCM).Given the quality control standards for the TCM decoction service as issued by Zhejiang and other regions, the systems of supervision and assessment remain incomplete.Authors of the paper introduced a project improvement team, composed of Chinese medicine pharmacy, Chinese medicine experience specialists, vendors of Chinese medicine decoction and express delivery companies, hence establishing a " three-in-one" supervision system of Internet+TCM decoction and delivery service. This practice can optimize the assessment indexes, strengthen the assessment system of assessment transformation and supervision system for patient medication.It proves that the practice contributes to higher quality and safety of TCM decoction and delivery service, improves the ability and level of TCM services, and ensures the medication safety of patients.
ABSTRACT
; Aim To study the synergistic inhibition effect of hydroxychloroquine (HCQ) and bevacizumab (B E V) on the cell growth of HUVECs and its mechanism. Methods RTCA,flow apoptosis assay and the vascularization assay of HUVECs were used to study the effect of HCQ combined with BEV on HUVEC proliferation, cell apoptosis and vascularization. The expressions of LC3 and p62 proteins were detected by Western blot, autophagy flow was observed through m Cherry EGFPLC3 double fluorescence, the changes of autophagosome and mitochondria were observed by electron microscopy, and the regulatory molecular mechanism of HCQ combined with BEV was revealed by transcriptome sequencing (RNA-seq). Results Compared with single drug groups, the combination of HCQ and BEV could synergistically inhibit HUVEC proliferation, promote HUVEC apoptosis, and inhibit the formation of vascular structures in HUVECs. HCQ could inhibit autophagy of HUVECs. Transcriptome sequencing results showed that the combination of HCQ and BEV influenced 69 different expressed genes,and the combination of HCQ and BEV might synergistically inhibit HUVECs by regulating "hub" genes such as RICTOR and PIK3CA. Conclusions The combination of HCQ and BEV can synergistically inhibit HUVECs, which is related to not only the inhibition of autophagy by HCQ, but also the regulation of " hub " genes such as RICTOR and PIK3CA.
ABSTRACT
Objective To study the effect of matrine on Calpain and MAP-2 in rats with experimental autoimmune encephalomyelitis(EAE).Methods In accordance with the random number table ,60 Wistar rats were divided into 6 groups randomly: normal group,model group,dexamethasone(DEX)-treated group(1mg/kg),high-dose matrine(MAT)-treated group(250mg/kg),middle-dose MAT-treated group(200mg/kg) and low-dose MAT-treated group(150mg/kg).The EAE models were induced by immunized spinal cord extracts of guinea pig with complete Freunds'adjuvant.Rats of three MAT-treated groups and DEX-treated group were injected intraper-itoneally with MAT and DEX daily for 16 days respectively,whereas rats of normal group and model group were injected intraperitoneally with normal saline.Clinical signs of rats in six groups were observed daily .Hematoxylin-eosin (HE) was used to analyze histopathological evaluation of spinal cord .μ-Calpain,m-Calpain and MAP-2 in spinal cord were determined using RT-PCR and immunohistochemistry respectively .Results Compared with the model group[(2.85 ±0.78)points],the clinical scores were significantly decreased in high-dose-MAT group[(1.28 ± 0.59) points], middle-dose-MAT group [(1.45 ±0.64) points] and low-dose-MAT group [(2.09 ± 0.71)points](t =5.345,4.314,2.869,all P <0.05).The HE score of rats in model group[(2.49 ±0.29)points] was significantly higher than that in high-dose-MAT group[(1.04 ±0.26) points],middle-dose-MAT group [(1.29 ±0.20) points] and low-dose-MAT group[(1.77 ±0.24)points] (t =5.185,4.274,3.629,all P <0.01).The levels of μ-Calpain mRNA and m-Calpain mRNA in the three MAT-treated groups were significantly lower than those in model group(t =10.656,9.418,7.044,all P <0.01;t =6.332,5.416,3.978,all P <0.01).In addition,the expression of MAP-2 in the spinal cord of EAE rats showed a marked elevation after MAT treatment (t =12.841,9.924,7.038,all P <0.01).Conclusion Matrine may be an effective therapeutic approach for EAE by inhibiting Calpain and increase MAP-2 expression.